Reverse-Transcription Loop-Mediated Isothermal Amplification and alternative protocols for lower cost, large-scale COVID-19 testing: lessons from an emerging economy / Amplificación isotérmica mediada por bucle de transcripción inversa y protocolos alternativos para pruebas COVID-19 a gran escala y de bajo costo: lecciones de una economía emergente
Rev. biol. trop
; 70(1)dic. 2022.
Article
En
| LILACS, SaludCR
| ID: biblio-1387718
:
CR1.1
Abstract
Introduction:
Most successful cases of COVID-19 pandemic mitigation and handling have relied on extensive reverse-transcription quantitative polymerase chain reaction (RT-qPCR). However, many emerging economies have struggled with current molecular testing demands due to economic, technical and technological constraints.Objective:
To define a potential diagnostic protocol to increase testing capacity in current and post-pandemic conditions.Methods:
We reviewed the literature, patents and commercial applications, for alternatives.Results:
We found a good potential in saliva samples, viral inactivation and quick RNA extraction by heating; the use of an isothermal technology such as loop mediated isothermal amplification (LAMP) and naked eye test-result visualization by in-tube colorimetry or turbidity.Conclusions:
Saliva samples with quick RNA extraction by heating and colorimetric LAMP are promising options for countries with economic and infrastructure limitations.
Resumen